@article {2048,
	title = {Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains.},
	journal = {AIDS research and human retroviruses},
	volume = {23},
	year = {2007},
	month = {2007 Dec},
	pages = {1593-8},
	abstract = {The HIV fusion inhibitor enfuvirtide (ENF/Fuzeon) targets the env gp41 transmembrane domain. Mutations in gp41 are associated with ENF resistance. We developed a prototype assay to genotype a 676-bp region spanning the heptad repeat domains (HR1 and HR2) of HIV-1 gp41. Plasma samples were collected from 126 HIV-1-infected blood donors in Cameroon, Brazil, Uganda, South Africa, Thailand, and Argentina. Based on analysis of gag p24, pol integrase, and env gp41 genes, the panel was composed of subtypes A/A2 (18), B (11), C (14), D (10), F/F2 (9), G (7), CRF01_AE (9), CRF02_AG (33), and recombinant strains (15). Genotyping was successful for 119 of the 126 samples (94.4\%). Although numerous amino acid polymorphisms were detected in some samples, none had primary mutations associated with ENF resistance. The gp41 HIV-1 research reagents developed by Celera are useful tools for genotyping analysis of the gp41 region in diverse HIV-1 strains.},
	keywords = {Base Sequence, Drug Resistance, Viral, Genotype, HIV Envelope Protein gp41, HIV Fusion Inhibitors, HIV Infections, HIV-1, Humans, Molecular Sequence Data, Peptide Fragments, Polymorphism, Genetic, Protein Structure, Tertiary, Sequence Alignment},
	issn = {0889-2229},
	doi = {10.1089/aid.2007.0130},
	author = {Eshleman, Susan H and Hudelson, Sarah E and Bruce, Robert and Lee, Travis and Owens, Michelle R and Hackett, John and Swanson, Priscilla and Devare, Sushil G and Marlowe, Natalia}
}
