| Title | C-type lectin Mermaid inhibits dendritic cell mediated HIV-1 transmission to CD4+ T cells. |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | Nabatov, Alexey A., Marein A. W. P. de Jong, Lot de Witte, Silvia Bulgheresi, and Teunis B. H. Geijtenbeek |
| Journal | Virology |
| Volume | 378 |
| Issue | 2 |
| Pagination | 323-8 |
| Date Published | 2008 Sep 1 |
| ISSN | 1096-0341 |
| Keywords | Animals, Anti-HIV Agents, CD4-Positive T-Lymphocytes, Cell Adhesion Molecules, Cell Line, Cells, Cultured, Dendritic Cells, Helminth Proteins, HIV Envelope Protein gp120, HIV-1, Humans, Lectins, C-Type, Nematoda, Protein Binding, Receptors, Cell Surface, Virus Attachment |
| Abstract | Dendritic cells (DCs) are important in HIV-1 transmission; DCs capture invading HIV-1 through the interaction of the gp120 oligosaccharides with the C-type lectin DC-SIGN and migrate to the lymphoid tissues where HIV-1 is transmitted to T cells. Thus, the HIV-1 envelope glycoprotein gp120 is an attractive target to prevent interactions with DCs and subsequent viral transmission. Here, we have investigated whether the structural homologue of DC-SIGN, the nematode C-type lectin Mermaid can be used to prevent HIV-1 transmission by DCs. Our data demonstrate that Mermaid interacts with high mannose structures present on HIV-1 gp120 and thereby inhibits HIV-1 binding to DC-SIGN on DCs. Moreover, Mermaid inhibits DC-SIGN-mediated HIV-1 transmission from DC to T cells. We have identified Mermaid as a non-cytotoxic agent that shares the glycan specificity with DC-SIGN and inhibits DC-SIGN-gp120 interaction. The results are important for the anti-HIV-1 microbicide development directed at preventing DC-HIV-1 interactions. |
| DOI | 10.1002/hpm.1029 |
| Alternate Journal | Virology |
