| Title | Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains. |
| Publication Type | Journal Article |
| Year of Publication | 2007 |
| Authors | Eshleman, Susan H., Sarah E. Hudelson, Robert Bruce, Travis Lee, Michelle R. Owens, John Hackett, Priscilla Swanson, Sushil G. Devare, and Natalia Marlowe |
| Journal | AIDS research and human retroviruses |
| Volume | 23 |
| Issue | 12 |
| Pagination | 1593-8 |
| Date Published | 2007 Dec |
| ISSN | 0889-2229 |
| Keywords | Base Sequence, Drug Resistance, Viral, Genotype, HIV Envelope Protein gp41, HIV Fusion Inhibitors, HIV Infections, HIV-1, Humans, Molecular Sequence Data, Peptide Fragments, Polymorphism, Genetic, Protein Structure, Tertiary, Sequence Alignment |
| Abstract | The HIV fusion inhibitor enfuvirtide (ENF/Fuzeon) targets the env gp41 transmembrane domain. Mutations in gp41 are associated with ENF resistance. We developed a prototype assay to genotype a 676-bp region spanning the heptad repeat domains (HR1 and HR2) of HIV-1 gp41. Plasma samples were collected from 126 HIV-1-infected blood donors in Cameroon, Brazil, Uganda, South Africa, Thailand, and Argentina. Based on analysis of gag p24, pol integrase, and env gp41 genes, the panel was composed of subtypes A/A2 (18), B (11), C (14), D (10), F/F2 (9), G (7), CRF01_AE (9), CRF02_AG (33), and recombinant strains (15). Genotyping was successful for 119 of the 126 samples (94.4%). Although numerous amino acid polymorphisms were detected in some samples, none had primary mutations associated with ENF resistance. The gp41 HIV-1 research reagents developed by Celera are useful tools for genotyping analysis of the gp41 region in diverse HIV-1 strains. |
| DOI | 10.1089/aid.2007.0130 |
| Alternate Journal | AIDS Res. Hum. Retroviruses |
