Yale University

Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains.

TitleAnalysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains.
Publication TypeJournal Article
Year of Publication2007
AuthorsEshleman, Susan H., Sarah E. Hudelson, Robert Bruce, Travis Lee, Michelle R. Owens, John Hackett, Priscilla Swanson, Sushil G. Devare, and Natalia Marlowe
JournalAIDS research and human retroviruses
Date Published2007 Dec
KeywordsBase Sequence, Drug Resistance, Viral, Genotype, HIV Envelope Protein gp41, HIV Fusion Inhibitors, HIV Infections, HIV-1, Humans, Molecular Sequence Data, Peptide Fragments, Polymorphism, Genetic, Protein Structure, Tertiary, Sequence Alignment
AbstractThe HIV fusion inhibitor enfuvirtide (ENF/Fuzeon) targets the env gp41 transmembrane domain. Mutations in gp41 are associated with ENF resistance. We developed a prototype assay to genotype a 676-bp region spanning the heptad repeat domains (HR1 and HR2) of HIV-1 gp41. Plasma samples were collected from 126 HIV-1-infected blood donors in Cameroon, Brazil, Uganda, South Africa, Thailand, and Argentina. Based on analysis of gag p24, pol integrase, and env gp41 genes, the panel was composed of subtypes A/A2 (18), B (11), C (14), D (10), F/F2 (9), G (7), CRF01_AE (9), CRF02_AG (33), and recombinant strains (15). Genotyping was successful for 119 of the 126 samples (94.4%). Although numerous amino acid polymorphisms were detected in some samples, none had primary mutations associated with ENF resistance. The gp41 HIV-1 research reagents developed by Celera are useful tools for genotyping analysis of the gp41 region in diverse HIV-1 strains.
Alternate JournalAIDS Res. Hum. Retroviruses

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